INTRODUCTION
THIS IS A PRE-SOLICITATION NON-COMPETITIVE (NOTICE OF INTENT) SYNOPSIS TO AWARD A CONTRACT OR PURCHASE ORDER WITHOUT PROVIDING FOR FULL OR OPEN COMPETITION (INCLUDING BRAND- NAME).

The National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), Office of Acquisitions (OA) on behalf of the National Institute of Digestive, Diabetes & Kidney Diseases (NIDDK) intends to award a purchase order without providing for full and open competition (Including brand-name) to BROAD INSTITUTE, INC for whole genome sequencing on 35 mouse genomes.

NORTH AMERICAN INDUSTRY CLASSIFICATION SYSTEM (NAICS) CODE
The intended procurement is classified under NAICS code 541380 with a Size Standard $15,000,000.

REGULATORY AUTHORITY
The resultant contract will include all applicable provisions and clauses in effect through the Federal Acquisition Circular (FAC) 2005-98

This acquisition is conducted under the procedures as prescribed in FAR subpart 13-Simplified Acquisition Procedures at an amount not exceeding the simplified acquisition threshold ($250,000).

STATUTORY AUTHORITY
This acquisition is conducted under the authority of 41 U.S.C. 253(c) under provisions of the statutory authority of FAR Subpart 6.302- FAR 6.302-1-Only one responsible source and no other supplies or services will satisfy agency requirements 41 U.S.C. 253(c)(1).

PERIOD OF PERFORMANCE
WITHIN 60 DAYS OF AWARD

Place of Performance
National Institutes of Health
National Institute of Digestive, Diabetes & Kidney Diseases
8 Center Drive
Bethesda MD 20892

DESCRIPTION OF REQUIREMENT
The Laboratory of Genetics and Physiology (NIDDK) conducts experiments aimed to mutate single bases in the mouse genome using an advanced gene editing technique called ‘base-editing'. To determine to what extent base editing affects not only its target sites in the genome but also unrelated stretches of DNA, we will conduct Whole Genome Sequencing (WGS).

These genomes are from mice in which a specific site within its DNA has been mutated using base editing. The (mutated) sites are responsible for the binding of a protein that regulates the expression of the respective gene in the mammary gland, liver and T cells.

The specific objective is to obtain the DNA sequence of 35 genomes from mice that carry a specific mutation introduced by base editing. The contractor needs to generate libraries from genomic DNA from 35 mice and conduct Whole Genome Sequencing.

Independently, and not as an agent of the Government, the contractor shall perform all of the detailed Task Areas listed below:

5.1 Task Area 1- Library Preparation
Library type: Illumina - DNA (whole genome)
Number of libraries: 35
Library prep kit: Kapa DNA library
Library preparation for WGS - KAPA Hyper Prep Kit

5.4 Task Area 4- Next Generation Sequencing
Illumina - HiSeq X - PE 150 Cycle
Read length: 2x150 (paired end)
Number of samples 35
Guaranteed output per sample: 60x mean coverage
Average coverage per sample: >65x mean coverage
Average PF bases per sample: >240 Gb
QC (Qubit, Tapestation, qPCR)

CLOSING STATEMENT
This synopsis is not a request for competitive proposals. However, interested parties may identify their interest and capability to respond to this notice. Responses to this notice shall contain sufficient information to establish the interested parties' bona-fide capabilities for fulfilling the requirement and include: descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all the foregoing requirements, the prompt payment discount terms, the F.O.B. Point (Destination or Origin), the Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. All offerors must have an active registration in the System for Award Management (SAM) www.sam.gov."

A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. The information received will normally be considered solely for the purposes of determining whether to proceed on a non-competitive basis or to conduct a competitive procurement.

All responses to this notice shall be submitted electronically by 9:00 am Eastern Standard Time, on Wednesday, August 8, 2018 to the Contract Specialist, Amber Harris, at [email protected] .

Assessment of Capability
Lowest Price Technically Acceptable