The Genomics and Pathogen Discovery Unit of the U.S. Naval Medical and Research Unit Six (NAMRU-6) is looking for reagents to prepare libraries using human and animal samples that later will be sequenced in the MiSeq platform developed by Illumina. Illumina is the sole supplier of reagents and consumables that are compatible with the MiSeq sequencer.
NAMRU-6 is looking for a library preparation kit that should include one step that contains an enzymatic fragmentation, size selection, end-repair, a-tail and ligation step in the same reaction. The process should allow including dual index at the 5 and 3 prime end for later sample identification. Also, the library preparation kit should be designed to construct libraries with a low DNA input (~50ng) with master-mixed reagents ready to use. Similarly, the sequencing kit should allow us to run more than 150 sequencing cycles. Those cycles are needed to run paired-end libraries with a high quality performance. We expect to have more than 2.0Gb per sequencing run with high Q30 values. Finally, the sequencing kit should include an internal control to validate the sequencing run.
The Nextera XT DNA sample preparation kit, the Index kit, PhiX control and the MiSeq reagent nano kit v2 are all Illumina reagents that meet our requirements for this purchase. All the reagents listed will be used with the MiSeq sequencer currently being used at NAMRU-6.
If capable sources are not identified through this notice, then NAMRU 6 intends to award a sole source contract under the authority of FAR 13.106-1(b)(i) to Illumina Inc.
This pre-solicitation is not a request for competitive proposals and no solicitation document exists for the requirement. Sources interested in responding to this notice are required to submit a capability statement that includes management and technical data and cost information, in sufficient detail and with convincing evidence that clearly demonstrates the capability to perform the required work. Capability statements shall not exceed 5 (8.5 x 11 inch) pages using a font size no smaller than 10-point. All capability statements received by the due date of this notice will be considered by the Government. A request for documentation or additional information or submissions that only ask questions will not be considered as an affirmative response. A determination by the Government not to compete based on responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement or to proceed with a sole source contract.
Capability statements are due by 12:00 PM Local Time, 29 August, 2016. Capability statements shall be submitted by e-mail ONLY as a Microsoft Word or Adobe PDF attachment to the following address: [email protected]. Please use subject line "VEI16113ILLUMINA".

Contracting Office Address:
3230 Lima Pl, Washington DC, 20521
United States

Primary Point of Contact.:
Martin Vasquez
[email protected]

 

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