The United States Department of Agriculture (USDA), Animal and Plant Health Inspection Service (APHIS), National Plant Germplasm Biotechnology Laboratory (NPGBL) located in Beltsville, MD intends to issue a sole source Purchase Order award to Life Technologies Corporation located in Grand Island, NY for Invitrogen Platinum Brand enzyme mixtures and other biological reagents listed below under the authority of FAR 6.302-1 and 41 U.S.C. 253(c)(1).

Platinum Taq DNA Polymerase (#10966-034)
Platinum Taq DNA Polymerase (#10966-026)
SuperScript III Platinum One-Step qRT-PCR Kit (#11732-020)
RNaseOUT Recombinant Ribonuclease Inhibitor (#10777-019)
10 mM dNTP Mix (#18427-088)
10 mM dNTP Mix (#18427-013)
TOPO TA Cloning Kit (# K4510-20)
SuperScript III One-Step RT-PCR System with Platinum Taq (#12574-018)
SuperScript III One-Step RT-PCR System with PlatinumTaq (#12574-026)
PureLink PCR Purification Kit (#K3100-01)
UltraPure DEPC-Treated Water (#750024)
M-MLV Reverse Transcriptase (#28025-013)
UltraPure Agarose (#16500500)
DynaMag-2 Magnet (#123-21D)
RPMI (#11875-093)
CO2 Independent Medium (#18045-088)
Blasticidin S HCl (#R21001)
Penicillin-Streptomycin-Glutamine (100X), Liquid (#10378-016)
MEM Non-Essential Amino Acids Solution 10 mM (100X), Liquid (#11140-050)
MEM Sodium Pyruvate Solution 100 mM (100X), Liquid (#11360-070)
Fungizone Antimycotic, Liquid (#15290-018)
HulaMixer Sample Mixer (#15920D)
imMedia Amp Agar (#Q60120)
imMedia Amp Liquid (#Q60020)

These reagents are used in specific, validated testing protocols for APHIS National Surveys and in the National Plant Germplasm Quarantine Unit. The validated testing protocols cannot be modified with any changes or substitutions of a critical reagent. To do so would result in negative consequences, such as weeks of expensive testing to establish alternative protocols. Any protocol modifications would also result in a halt to ongoing processing of National Survey samples. No other brand reagents are available to meet the NPGBL testing requirement.

A one-time purchase order will be awarded with an estimated value of $65,000.00. This notice is for informational purposes only. No formal solicitation will be issued. Any questions or inquiries must be mailed, e-mailed or faxed to the attention of the Contracting Specialist listed below as the point of contact in this notice.

Point of contact: Michael Chiodi, Contracting Specialist, (612) 336-3203, FAX: (612) 336-3550, E-mail: [email protected].

 

 

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