The purpose of Amendment 1 to DARPA-BAA-16-42, the Prometheus Broad Agency Announcement, is to revise the Program Structure and Technical Area 1 description as shown below and incorporated into the Amendment 1 Full BAA package.  Changes are underlined below.

The Prometheus Program Structure (Section 1.1, page 5) is revised as follows:

The DARPA Prometheus program seeks to advance our understanding of host molecular features that are predictive of infectious disease spreading potential by (1) collecting data, both retrospective and prospective, from relevant human respiratory infectious disease models, and (2) performing advanced, integrative analytics and predictive modeling to discover the molecular nature of host transmission potential and symptomatic response to pathogen. The program consists of two Technical Areas with a total period of performance of 14 months: Technical Area 1 (TA 1) - Host molecular target discovery, Technical Area 2 (TA 2) - Prognostic algorithm development.

Proposers may propose to one or both Technical Areas. Proposals that include both Technical Areas are strongly encouraged.

The Description of Technical Areas (Section 1.1, page 7) is revised as follows:

Aim 1. Retrospective data analysis
Performers will collect publically available retrospective data, and/or previously unreleased data generated by the performer, consisting of molecular profiles across multiple time points from peripheral blood of humans with known exposure to a respiratory virus. These molecular profiles can be generated using various levels of omics techniques, which may include gene expression by microarrays or mRNA-Seq, micro-RNA profiling using miRNA-Seq or microarray platforms, proteomics, and metabolomics. The objective is to collect, curate, and analyze data from at least 100 individuals (milestone n = 100 at three months).