Contracting Activity:USDA-ARS St Paul, Minnesota intends to Award as a Sole Source to Illumina.

Nature/Description of Action: Request for purchasing laboratory consumables for conducting genotyping experiments using the high throughput genotyping assay method developed by Illumina.

Description of Supplies/Service:The supplies required are eight units from Illumina Inc. of Infinium iSelect Custom Kit for 48 DNA samples for genetic analysis of 90,000 loci in wheat. This order is for genetic analysis with SNP markers for a total of 384 DNA Samples.The supplies required are eight units from
Illumina Inc. of Infinium iSelect Custom Kit for 48 DNA samples for genetic analysis of 90,000 loci in wheat. This order is for genetic analysis with SNP markers for a total of 384 DNA Samples

Statutory Authority: In accordance with 41 U.S.C. 253(c) and FAR 6.302-1.

Demonstration of Contractor's Unique Qualifications:.. Illumina GoldenGate genotyping is a high-density genotyping assay that allows a large amount of data to be generated in a short amount of time. The assay requires reagents and materials designed and synthesized according to sequencing data. Sequencing data will be available upon award. Items are described as follows:

a. Manufacturer's Part Number: WG-401-1001 12each ,Infinium iSelect HD Custom Kit contains reagents for processing 48 DNA samples and analyzing 3,072 to 90,000 loci. Species: Wheat

Determination of Fair and Reasonable Costs:
The Illumina genotyping technique is designed for high throughput genotyping experiments. The item stated part b. above is provided at a 5.59% discount.

Market Survey:The item stated above is manufactured and custom made at Illumina. Illumina developed the GoldenGate assay method and, thus, is the sole manufacturer of these chemical reagents for the assay, and no substitute vendor is available.