Please quote the following including all shipping and handling fees.

1. QTY. 1 [SPRCH-01006] Purchase BAC from CHORI - BACs purchased from CHORI - Turnaround time: 4-5 weeks - Shipped and handled by CHORI

2. QTY. 1 [SGMMU-08001] LFKI, any Locus - Conventional Vector Construction - Turnaround time: 8-12 weeks - Deliverable: Any Locus Large-fragment Knockin Vector (bacterial stab) and Vector Construction Report - Design and construction of any locus large-fragment knockin vector based on client-approved strategy. NOTE: Pricing/turnaround time applies to projects with fragments up to 4kb in length. Larger fragments can be accommodated at an additional cost and modified guarantee.

3. QTY. 1 [SGMMU-08003] LFKI, any locus - CRISPR/Cas9 injection to obtain knockin founders - Turnaround time: 8-18 weeks - Deliverable: Any Locus Large-fragment Knockin Founders and Nuclease Injection Report. - The nuclease mRNA and any locus large-fragment vector will be co-injected into fertilized mouse eggs, followed by implantation of the eggs into surrogate mothers to obtain offspring. Cyagen will inject as many eggs and/or target as many sites as needed to fulfill the guarantee.

4. QTY. 1 [SGMMU-08004] LFKI, any locus - Genotyping pups to identify founders - Turnaround time: 1-2 weeks - Pups will be screened by PCR to identify any locus large-fragment knockin founder mice.

5. QTY. 1 [SGMMU-08005] LFKI, any locus - F1 Heterozygotes Generation - Turnaround time: 12-16 weeks - Deliverable: a minimum of three F1 heterozygote animals and Breeding report - Cyagen will breed the founders to wildtype mice of matching strain background, and genotype their offspring to obtain F1 animals.

6. QTY. 1 [SPRCH-01009] Handling - Full Gene Targeting Project (one line only) - Handling fee for 1 rodent crate.