The USDA, Agricultural Research Service, Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska, intends to enter into a single source contract with Roche Diagnostics, Indianapolis, Indiana, for reagents. The reagents to be purchased are 2 kit-05 608 228 001
GS Rapid Library Prep Kit; 1 kit-05 463 343 001
Titanium Paired End Adapter Kit; 8 kit-05 618 428 001 GS Titanium LV emPCR Kit (Lib-L) - emPCR kit V2; 1 kit-05 618 436 001 GS Titanium MV emPCR Kit (Lib-L) - emPCR kit V2; 4 kit-05 618 444 001 GS Titanium SV emPCR Kit (Lib-L)- emPCR kit V2; 9 kit-06 380 565 001
GS Titanium Sequencing Kit XLR70+ - (400 cycle -70 x 75) For use with the GS Titanium PicoTiterPlate Kit 70x75; 10 kit-05 233 682 001 GS Titanium PicoTiterPlate Kit 70x75 - PTP with unique metallic coating to be used only with Titanium emPCR and Titanium Sequencing Kit XLR70. Plate uses 2-, 4-, 8- and 16-region gaskets; 1 kit - 05 233 658 001 GS Titanium emPCR Breaking Kits MV/ LV 12p; 1 ea 05 233 674 001 GS Titanium emPCR Filters SV 64pc; 4 kit - 04 932 358 001 GS FLX - Maintenance Wash Kit; 1 ea - 05 414 601 001 70X75 BEAD DEP DEVICE 2 REGION TH

We need reagents and supplies to support running a Genome Sequencer FLX system housed at the U.S. Meat Animal Research Center. The Genome Sequencer FLX instrument (GS FLX) is a DNA sequencer designed and built by 454 Life Sciences and distributed by Roche Applied Science. The technology for this system is based on enrichment of fragmented DNA followed by clonal amplification and pyrosequencing. The sequencer can generate up to 500 million bases per 7.5 hour run, achieve reads averaging 350 to 450 bases, attain over 1,000,000 reads per run, generate single-read accuracy that is greater than 99%, and achieve consensus accuracy greater than 99.99%. Reagents required to run this system include GS Titanium compatible library preparation kits for linker modified single stranded DNA, SV emulsion based PCR kits for titration of the library fragments for the sequencing reaction, LV emulsion based PCR kits for preparative reactions of bead-linked DNA fragments for sequencing, Titanium chemistry sequencing kits, and PicoTiter Plate Kits which provide the surface for sequencing of the DNA fragments.
Roche is the sole supplier and distributor of the GS FLX instrument. The GS titanium library preparation reagents, emulsion based PCR kits, sequencing kits, and PicoTiter Plate Kits are only available through Roche. The GS FLX warranty is conditional that customers agree to use the instrument in strict accordance with Roche operating instructions, including Roche sole source specifications regarding reagent kits. The FLX sequencer makes use of very specialized reagents only available from Roche at the present time in a form suitable for use on the instrument.

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