The National Institute of Digestive, Diabetes & Kidney Diseases's (NIDDK) requires a Gatan 626 Cryo-holder and its accessories for screening cryo-em grids. To study protein structures by Cryo-EM, the proteins are embedded in vitreous ice to preserve their most native structures, and they need to be maintained at liquid nitrogen temperature during observation by electron microscope. The Gatan cryo holder can hold liquid nitrogen and keep frozen protein samples at liquid nitrogen temperature for imaging by electron microscope. The Cryo-EM images will be used for reconstruction of 3D structures of proteins. The Gatan 626 Cryo holder has a small Dewar to hold liquid nitrogen, so as to maintain the Cryo-EM grids loaded onto the holder at the liquid nitrogen temperature. The vitreous ice embedded protein samples on the Cryo-EM grids thus can be kept at Cryo temperature to preserve their structure and for Cryo-EM imaging. This new Gatan 626 Cryo holder is compatible with all other equipment and tools which the NIDDK has, such as the pumping station holder holding station tube diameter, temperature controller software etc. In addition, there are another two Gatan 626 cryo holders in NIDDK Cryo-EM facility and their accessories (pumping stations, temperature controllers, ample loading clip tools, sample loading stations etc). The pumping station for this new cryo holder will also work as a replacement for an already owned broken pumping station so it can be used with other already owned same model cryo-holders. This new holder will be used on the NIDDK Cryo-EM facility microscope for MICEF users only. It can share the same supporting items and accessories which we already have.
The specs are as follows:
Turbo Pumping Station-- Benchtop pumping station that includes work column, CC gauge tube, one TEM holder module, and one 655.05300 shut-off valve.
Single Tilt Liquid Nitrogen CryoTransfer Holder-- Designed for low temperature transfer and subsequent recording of radiation-sensitive, frozen-hydrated specimens for cryotransmission electron microscopy (cryo-EM); Includes Workstation and Tools Capabilities and performance specifications
Temperature Controller, Model 1905-- Temperature controller for heating, cooling and cryo-holders
TEM Holder Module-- TEM holder module for turbo pumping station
Shut-off valve, required for pumping cryoholder dewar
The proposed contract action is for supplies or services for which the Government intends to solicit and negotiate with only one source under authority of the 41 U.S.C. 253(c) (1), FAR 6.302. Only one responsible source and no other supplies or services will satisfy agency requirements.
The offeror must include a completed copy of the provision of FAR Clause 52.212-3, Offeror Representations and Certifications - Commercial Items with its offer. The provisions of FAR Clause 52.212-1 Instructions to Offerors - Commercial Items; FAR Clause 52.212-2, Evaluation - Commercial Items; FAR Clause 52.212-4, Contract Terms and Conditions - Commercial Items; and FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders - Commercial Items - Deviation for Simplified Acquisitions applies to this acquisition. The offeror must include their Dun & Bradstreet Number (DUNS), the Taxpayer Identification Number (TIN), and the certification of business size. The clauses are available in full text at http://www.acquisition.gov/far/. Interested vendors capable of furnishing the government with the services specified in this synopsis should submit a copy of their quotation to the below address or via email to [email protected]. Offers must also be accompanied by descriptive literature, delivery timeframe, warranties and/or other information that demonstrates that the offer meets all the foregoing requirements. Quotations will be due on or before August 14, 2017 by 10 am, EST. via email or postal mail. The quotation must reference "Solicitation number" NIH-NIDDK-17-215. Quotations sent by postal mail or other mailing services must be submitted to the following address: Eunice Kennedy Shriver National Institute of Child Health and Human Development, 6710B Rockledge Drive, Room 1159A, Bethesda, MD 20817. Attention: Amber Harris, by the date and time mentioned above. Any questions must be sent via email to [email protected] and must include solicitation#NIH-NIDDK-17-215 in the subject line of email. Faxed copies/responses will not be accepted.
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