The vendor shall provide an equipment of brand name or equal specification to the Bio-Rad Quest 10 Plus Chromatography System and a BioFrac Fractionation System. Equipment installation, software and 12 months warranty that includes parts and labor must be provided.   NIAMS is seeking to acquire  a fast protein liquid chromatography instrument of brand name or equal specification to the NIAMS Bio-Rad Quest 10 Plus Chromatography and BioFrac Fractionation Systems.

In order to support existing research projects, the laboratory requires a Fast Protein Liquid Chromatography instrument (FPLC) with the following

characteristics:

1. Standalone with small footprint protein purification apparatus
2. Allow for the purification of proteins, RNPs, and RNAs
3. Be capable of handling multiple protein purification strategies, including gel filtration, IMAC, or ion-exchange chromatography
4. Be programmable, automated operation
5. Supports automated fractionation collection
6. Be capable of conductive measurements
7. Be modular for future expansions/increased capability
8. Includes a high-resolution touchscreen to visualize results
9. Create an accurate blended buffer gradient
10. Allow flexible placement of valves and detectors to minimize flow path
11. Allow user friendly setup of connections, e.g. by LED-guided plumbing
12. Allow user-friendly extension and addition of modules, ideally through automated module discovery and easy application-based system setup
13. Include a multiwavelength detection module for simultaneous four-wavelength detection at 190–800 nm (UV/VIS)
14. Automated 10 ml/min pumps
15. Supports flow rates of 0.001–10 ml/min at 3,650 psi
16. Injection valve must multiple volume injections (263 to 750 microliters)
17. Include a pre-installed machine control and analysis software
18. Software must support instrument control, data collection and analysis

  OTHER REQUIREMENTS:

 Twelve months warranty

1. PC Computer with 22-inch monitor
2. Software License
3. Instrument and Proteomics Training
4. Microplate Rack with ice bath that holds 120 tubes, 12--13 mm in diameter; 4 SBS format microplates (96-, 48-, 24-, or 12-wells); Titertube and microtubes (8 x 12, 96-tubes)