The Laboratory of Cellular and Developmental Biology of the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase a BD Accuri C6 Plus flow cytometry system.  

The contractor shall provide the following to brand name specifications:

The features of the BD Accuri C6 Plus that meet our critical needs are as follows:

• • • 7.2-decade dynamic range (16 million channels of digital data)
    • Highly sensitive: MESF <75 (FITC) and <50 (PE)1
    • Non-pressurized peristaltic pump system
    • Event rates up to 10,000 events per second
    • Small footprint
    • Uses laboratory-grade water as sheath fluid
    • Blue and red laser, two light scatter detectors, and four fluorescence detectors
    • with optical filters optimized for the detection of fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes
    • Analyzes variants of fluorescent proteins, such as GFP, YFP, and mCherry.
    • Software allows visualization of data at any scale, so that users can precisely set gates and regions.
    • Can calculate absolute counts or sample concentration per μL without the use of counting beads
    • Automated sampler that supports
• 48-well plates
• 96-well plates
• 96-deep-well plates
• 24-tube rack (included) for standard 12 x 75-mm tubes
• Software features free downloadable software templates for many        assays and applications
• Automated instrument quality control
• Analyzes a variety of cellular processes:
• Apoptosis
• Cell signaling
• DNA content
• DNA damage
• Cell cycle
• Proliferation
• Viability

Facilitates multiple aspects of stem cell research from the assessment of

pluripotent and adult stem cell phenotypes to iPSC reprogramming efficiency.