The USDA, Agricultural Research Service, Biosciences Research Laboratory, Fargo, North Dakota, intends to enter into a sole source contract with Illumina, Inc., San Diego, California, for the purchase of consumables (Resynthesis of Barley OPA) for a Government-owned Illumina BeadStation 500 GX and includes: 1. Custom Oligo Assay Pool (192 samples, 1536-plex) Single Locus, including oligonucleotide design, synthesis and pooling on a per locus basis, supports 192 samples for 1536 SNP loci; 2. Sentrix Universal-96 Array Matrix (1536-plex), a single Sentrix Array Matrix that can process 96 samples with up to 1536 assays/sample; 3. Single-Use Activation Kit (576 Samples) for activating DNA for use with the Genotyping Kit with enough reagents for six 96-well plates of samples for a single genotyping reaction (250 ng of input DNA required); and 4. GoldenGate Assay Kits (96 samples) for preparing genotyping reactions for 96 samples using the GoldenGate Assay. This system is unique in that the assay will be carried out using a pool of 1536 oligomers against 96 barley samples done in parallel. One can perform 96 x 1,536=147,456 individual reactions simultaneously using this system. The reagents and the oligomer pool have to be custom made by Illumina for use with their system. *Provide Qualification Statement by Close of Business November 6, 2006, if interested in this procurement.
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