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Buyer: NIH Clinical Center 10 Center Drive rm2C527 Bethesda MD 20892 Reference Number: CC263-05-Q-MF-0544 Required By: 08-12-05 Delivery By: 08-16-05 Delivery FOB: Y Small Business Set-aside: Y FAR: 52.219-1 SMALL BUSINESS CONCERN REPRESENTATION 84041 SIC: 3841 FSC: 6515 Simplix Key: 8BEGUIO0 Delivery Destination: National Institutes Health; 9000 Rockville Pike; Bethesda, Md 20892 LINE ITEM 01: 1 Each This requirement is needed for a research laboratry /standin g purchase order with 24hr turn around delivery of orders un til funds are exhausted LINE ITEM 02: 1 Each one step pcr kits high fidelity taq polymerase LINE ITEM 03: 1 Each one step rt-pcr kits with high fidelity taq polymerase LINE ITEM 04: 1 Each tissue culture media including RPMI, Dmem high and low gluco se and eagles LINE ITEM 05: 1 Each tissue culture media supplements including high quality feta l bovine serum, glutamine,pen/strep antibiotics, growth supp lements, cell dissosiation buffers LINE ITEM 06: 1 Each gels and solutions fro the x-cell sure lock gel and blotting apparatus currently in use in the lab LINE ITEM 07: 1 Each various enzymes for molecular biology including restriction enzymes LINE ITEM 08: 1 Each kits for isolation of mRNA and genomic DNA LINE ITEM 09: 1 Each custom primers ssythesis for PCR and sequencing LINE ITEM 10: 1 Each cell seperation technology for negative and positve selectio n of various lymphocyte populations LINE ITEM 11: 1 Each detection kits for western blotting and immunohistochemistry of tissue samples ADDITIONAL PROVISIONS AND REPRESENTATIONS: Please fax quote to 301-480-1149

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